TNR16 Cell Based ELISA Kit (A103137)

TNR16 Cell Based ELISA Kit

TNR16 Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of p75NTR levels in adherent and suspension cells.

Cell-based (qualitative)

This TNR16 Cell Based ELISA Kit allows for the detection of TNR16 and the effects that certain stimulation conditions have on TNR16 expression in different cell lines. Qualitative determination of TNR16 concentration is achieved by an indirect ELISA format. In essence, the TNR16 is captured by Anti-TNR16 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TNR16 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Tissue Culture Treated Microplate

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

CD271, Gp80-LNGFR, Low affinity neurotrophin receptor p75NTR, Low-affinity nerve growth factor receptor, Low-affinity nerve growth factor receptor p75NGFR, Low-affinity nerve growth factor receptor p75NGR, NGF receptor, NGFR, p75 ICD, TNFRSF16, Tumor necrosis factor receptor superfamily member 16

Information online is representative. Always refer to the Product Manual inside the kit.