TRI18 Cell Based ELISA Kit (A103129)

TRI18 Cell Based ELISA Kit

TRI18 Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of MID1 levels in adherent and suspension cells.

Cell-based (qualitative)

This TRI18 Cell Based ELISA Kit allows for the detection of TRI18 and the effects that certain stimulation conditions have on TRI18 expression in different cell lines. Qualitative determination of TRI18 concentration is achieved by an indirect ELISA format. In essence, the TRI18 is captured by Anti-TRI18 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TRI18 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Tissue Culture Treated Microplate

Adherent and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 3-6 months.

E3 ubiquitin-protein ligase Midline-1, FXY, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, RING-type E3 ubiquitin transferase Midline-1, RNF59, TRIM18, Tripartite motif-containing protein 18, XPRF

Information online is representative. Always refer to the Product Manual inside the kit.