TSC22D1 Cell Based ELISA Kit (A102977)

TSC22D1 Cell Based ELISA Kit

Cell-based (qualitative)

This TSC22D1 Cell Based ELISA Kit allows for the detection of TSC22D1 and the effects that certain stimulation conditions have on TSC22D1 expression in different cell lines. Qualitative determination of TSC22D1 concentration is achieved by an indirect ELISA format. In essence, the TSC22D1 is captured by Anti-TSC22D1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TSC22D1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.

Microplate

Adherent cells and suspension cells.

> 5000 Cells

4 hours 30 minutes

Human, Mouse, Rat

Store at + 4°C. Stable for 6 months.

Cerebral protein 2, HUCEP-2, KIAA1994, Regulatory protein TSC-22, TGFB-stimulated clone 22 homolog, TGFB1I4, Transforming growth factor beta-1-induced transcript 4 protein, TSC22, TSC22 domain family protein 1

Information online is representative. Always refer to the Product Manual inside the kit.