Uncoated Rat IL1R1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay designed for the in vitro quantitative determination of rat IL1R1 in serum and plasma. Uncoated ELISA kits provide the essential components required to perform ELISA assays. They are an ideal cost-effective option for experienced users who can independently establish and optimize their own ELISA systems.
Assay Type
Sandwich (quantitative)
Principle of Assay
The Uncoated Rat IL1R1 ELISA Kit (A327170) is based on a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of IL1R1 in rat serum and plasma. In this format, a capture antibody specific to IL1R1 is first coated onto the wells of a 96-well microtiter plate by the user. After blocking nonspecific binding sites, standards or test samples are added, and IL1R1 present in the samples binds to the immobilized capture antibody. Following incubation, a biotinylated detection antibody specific for a different epitope of IL1R1 is introduced, forming a capture antibody–antigen–detection antibody complex. After washing to remove unbound detection antibody, HRP–streptavidin conjugate is added, binding to the biotinylated detection antibody. Unbound conjugate is removed by washing, and TMB substrate is added. The HRP enzyme catalyzes the conversion of TMB to a blue product, which changes to yellow after the addition of an acidic stop solution. The color intensity, measured at 450 nm, is directly proportional to the concentration of IL1R1 in the sample. Sample concentrations are determined by comparing their absorbance values to a standard curve generated from known IL1R1 standards.
Platform
Un-coated Microplate (12 x 8 well strips)
Detection Type
Colormetric
Instrument
Colorimetric Microplate Reader
Sample Type
Serum and plasma.
Product Range
78.125-5000 pg/ml
Assay Time
24 hours
Sample Size
100µl
Reactivity
Rat
Cross Reactivity
No significant cross-reactivity or interference between rat IL1R1 and analogues was observed.
Precision
Intra-assay CV: < 10%, Inter-assay CV: < 10%
Storage
Shipped at +4°C. Store short term at +4°C and long term at -20°C. Refer to Protocol Manual. Avoid freeze/thaw cycles.
Synonyms
CD121 antigen-like family member A, CD121a, IL-1R-1, IL-1R-alpha, IL-1RT-1, IL-1RT1, IL1R, IL1RA, IL1RT1, Interleukin-1 receptor alpha, Interleukin-1 receptor type 1, Interleukin-1 receptor type I, p80
Product Note
Information online is representative. Always refer to the Product Manual inside the kit.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
