Universal Bevacizumab/Avastin ELISA Kit (A326420)

Universal Bevacizumab/Avastin ELISA Kit

Universal Bevacizumab/Avastin ELISA Kit is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of universal Bevacizumab (Avastin), a therapeutic monoclonal antibody, in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. The assay is suitable for pharmacokinetic studies, bioanalytical testing, therapeutic antibody research, and biotherapeutic development.

Indirect (quantitative)

Universal Bevacizumab/Avastin ELISA Kit (A326420) is a 3 hour indirect Enzyme-Linked Immunosorbent Assay (iELISA) designed for the in vitro quantitative determination of universal Bevacizumab (Avastin), a therapeutic monoclonal antibody, in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Bevacizumab (Avastin) present in the samples binds to the immobilized antigen. Following incubation, unbound components are removed by washing and an HRP-conjugated detection antibody is added to the wells. This detection antibody binds the captured Bevacizumab (Avastin) to form an indirect immune complex. After further incubation and washing, TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Bevacizumab (Avastin) captured in each well. The concentration of Bevacizumab (Avastin) is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

0.313-20 ng/mL

3 hours

Universal

Specific for Bevacizumab (Avastin) with no reported cross-reactivity with other analogs or variants.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.