Principle of Assay
Universal C13orf8 ELISA Kit (A350416) is a 1 hour competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of universal C13orf8 in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with an antibody specific for C13orf8. During the assay, C13orf8 present in the standards or samples competes with HRP-labelled C13orf8 for binding to the immobilized capture antibody. Following incubation, unbound components are removed by washing and TMB substrate solution is added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the colour is measured at 450 nm using a microplate reader. The concentration of C13orf8 in the samples is inversely proportional to the OD450 value and is determined by comparison with the standard curve.