Universal Anti-CD200R1 Antibody [Janssen patent] ELISA Kit (A351077)

Universal Anti-CD200R1 Antibody [Janssen patent] ELISA Kit

Universal Anti-CD200R1 Antibody [Janssen patent] ELISA Kit is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of universal Anti-CD200R1 Antibody [Janssen patent] in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

Sandwich (quantitative)

Universal Anti-CD200R1 Antibody [Janssen patent] ELISA Kit (A351077) is a 4 hour sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of universal Anti-CD200R1 Antibody [Janssen patent] in serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids. A 96-well microtiter plate has been pre-coated with assay-specific antigen. Standards and samples are added to the wells, where Anti-CD200R1 Antibody [Janssen patent] binds to the immobilized antigen. Following incubation, unbound components are removed by washing and a biotinylated antigen detection reagent is added to the wells. This reagent binds the captured Anti-CD200R1 Antibody [Janssen patent] to form an antigen-antibody-antigen sandwich complex. After further incubation and washing, HRP-Streptavidin conjugate is added to the wells. TMB substrate solution is then added to visualize the HRP enzymatic reaction. This produces a blue-coloured product that changes to yellow following addition of acidic stop solution. The intensity of the yellow colour is directly proportional to the amount of Anti-CD200R1 Antibody [Janssen patent] captured in each well. The concentration of Anti-CD200R1 Antibody [Janssen patent] is calculated by reading the absorbance at 450 nm and comparing the values with the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric Detection (OD450)

Colorimetric Microplate Reader

Serum, plasma, cell culture supernatant, cell or tissue lysate, and other biological fluids.

1.563-100 ng/mL

4 hours

Universal

Demonstrates specificity for anti-CD200R1 antibodies [Janssen patent] with no reported cross-reactivity to related analogs.

Shipped at +4°C. Store kit components at +4°C or -20°C as instructed in the Protocol Manual. Do not use past expiration date! Avoid freeze/thaw cycles.

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.