Universal HRP Conjugated Streptavidin ELISA Kit (A326620)

Universal HRP Conjugated Streptavidin ELISA Kit

Universal HRP Conjugated Streptavidin ELISA Kit is a direct Enzyme-Linked Immunosorbent Assay (dELISA) designed for the in vitro quantitative determination of universal HRP Conjugated Streptavidin in serum, plasma and other biological fluids.

Direct (quantitative)

HRP Conjugated Streptavidin ELISA Kit (A326620) employs the direct enzyme immunoassay technique for the quantitative measurement of universal HRP Conjugated Streptavidin in serum, plasma and other biological fluids. The 96-well microtiter plate is pre-coated with HRP Conjugated Streptavidin antigen. Test samples and standards are added to the wells and incubated. After washing, an HRP-conjugated detection antibody specific for HRP Conjugated Streptavidin is added and incubated. Following a final wash, TMB substrate solution is used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of HRP Conjugated Streptavidin bound on the plate. The concentration of HRP Conjugated Streptavidin can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Pre-coated Microplate (12 x 8 well strips)

Colorimetric

Colorimetric Microplate Reader

Serum, plasma and other biological fluids.

14 µIU/ml

1 hour

Universal

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Store at +4°C. Do not use past expiration date!

Information online is representative. Always refer to the Product Manual inside the kit.

This product is for research use only. It is not intended for diagnostic or therapeutic use.