ZAP-70 (phospho Tyr315) Cell Based ELISA Kit is a Enzyme-Linked Immunosorbent Assay designed for the indirect in vitro quantitative determination of ZAP70 levels in adherent and suspension cells.
Assay Type
Cell-based (qualitative)
Principle of Assay
This ZAP-70 (phospho Tyr315) Cell Based ELISA Kit allows for the detection of ZAP-70 (phospho Tyr315) and the effects that certain stimulation conditions have on ZAP-70 (phospho Tyr315) expression in different cell lines. Qualitative determination of ZAP-70 (phospho Tyr315) concentration is achieved by an indirect ELISA format. In essence, ZAP-70 (phospho Tyr315) is captured by Anti-ZAP-70 (phospho Tyr315) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-ZAP-70 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.
Western Blot - ZAP-70 (phospho Tyr315) Cell Based ELISA Kit (A103782)
The mouse monoclonal antibody to GAPDH is used as a positive control in ZAP-70 (phospho Tyr315) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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