ZAP-70 (phospho Tyr315) Cell Based ELISA Kit (A103782)

ZAP-70 (phospho Tyr315) Cell Based ELISA Kit

Cell-based (qualitative)

This ZAP-70 (phospho Tyr315) Cell Based ELISA Kit allows for the detection of ZAP-70 (phospho Tyr315) and the effects that certain stimulation conditions have on ZAP-70 (phospho Tyr315) expression in different cell lines. Qualitative determination of ZAP-70 (phospho Tyr315) concentration is achieved by an indirect ELISA format. In essence, ZAP-70 (phospho Tyr315) is captured by Anti-ZAP-70 (phospho Tyr315) Antibodies while Dye 1-conjugated and Dye 2-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the dye conjugated to the secondary antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. Anti-ZAP-70 Antibody is also included for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

Microplate

> 5000 Cells

Human, Mouse

Store at + 4°C. Stable for 6 months.

70 kDa zeta associated protein, 70 kDa zeta-associated protein, 70 KDa Zeta-Associated Protein2, 70 KDa Zeta-Chain Associated Protein, EC 2.7.10, EC 2.7.10.2, FLJ17670, FLJ17679, Selective T cell defect, SRK, STCD, STD, Syk related tyrosine kinase, Syk-related tyrosine kinase, Truncated ZAP kinase, Tyrosine protein kinase ZAP70, Tyrosine-protein kinase ZAP-70, TZK, ZAP 70, ZAP70, ZAP70_HUMAN, Zeta chain associated protein kinase 70kD, Zeta chain associated protein kinase 70kDa, Zeta chain associated protein kinase 70kDa isoform 1, Zeta chain associated protein kinase 70kDa isoform 2, Zeta chain of T cell receptor associated protein kinase 70, Zeta chain TCR associated protein kinase 70kD, Zeta chain TCR associated protein kinase 70kDa, Zeta-Chain (TCR) Associated ProteinKinase (70 KD), Zeta-Chain Associated Protein Kinase, 70kD

Information online is representative. Always refer to the Product Manual inside the kit.