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Anti-14-3-3 beta + zeta (phospho Ser184 + Ser186) Antibody (A95648)

Western Blot - Anti-14-3-3 beta + zeta (phospho Ser184 + Ser186) Antibody (A8001) - Antibodies.com
$190
Product Datasheet

Rabbit polyclonal antibody to 14-3-3 beta + zeta (phospho Ser184 + Ser186) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 6-8 business days.

  • Specifications

Name
Anti-14-3-3 beta + zeta (phospho Ser184 + Ser186) Antibody
Description
Rabbit polyclonal antibody to 14-3-3 beta + zeta (phospho Ser184 + Ser186).
Specificity
This antibody detects endogenous levels of 14-3-3 beta/zeta only when phosphorylated at Ser184/186.
Applications
WB, ELISA
Dilutions
WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:5000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human 14-3-3 beta/zeta around the phosphorylation site of Ser184/186 (amino acids 151-200).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
27kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-14-3-3 beta + zeta (phospho Ser184 + Ser186) Antibody (A8001) - Antibodies.comEnlarge Image

Western Blot - Anti-14-3-3 beta + zeta (phospho Ser184 + Ser186) Antibody (A95648)

Western blot analysis of lysates from RAW264.7 cells treated with UV 15' using Anti-14-3-3 beta + zeta (phospho Ser184 + Ser186) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
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