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Anti-14-3-3 eta/YWHAH Antibody (A89060)

Western Blot - Anti-14-3-3 eta/YWHAH Antibody (A89060) - Antibodies.com Immunofluorescence - Anti-14-3-3 eta/YWHAH Antibody (A89060) - Antibodies.com Immunofluorescence - Anti-14-3-3 eta/YWHAH Antibody (A89060) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to 14-3-3 eta/YWHAH for WB, IHC and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-14-3-3 eta/YWHAH Antibody
Description
Rabbit polyclonal antibody to 14-3-3 eta/YWHAH.
Applications
WB, IHC, ICC/IF
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAH (NP_003396.1).
Sequence
MGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-14-3-3 eta/YWHAH Antibody (A89060) - Antibodies.comEnlarge Image

Western Blot - Anti-14-3-3 eta/YWHAH Antibody (A89060)

Western blot analysis of extracts of various cell lines, using Anti-14-3-3 eta/YWHAH Antibody (A89060) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Immunofluorescence - Anti-14-3-3 eta/YWHAH Antibody (A89060) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-14-3-3 eta/YWHAH Antibody (A89060)

Immunofluorescence analysis of NIH-3T3 cells using Anti-14-3-3 eta/YWHAH Antibody (A89060) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-14-3-3 eta/YWHAH Antibody (A89060) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-14-3-3 eta/YWHAH Antibody (A89060)

Immunofluorescence analysis of NIH-3T3 cells using Anti-14-3-3 eta/YWHAH Antibody (A89060) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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