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Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244)

Western Blot - Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244) - Antibodies.com Immunofluorescence - Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC1358] antibody to 14-3-3 Theta/Tau for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-14-3-3 Theta/Tau Antibody [ARC1358]
Description
Rabbit monoclonal [ARC1358] antibody to 14-3-3 Theta/Tau.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 14-3-3 Theta (P27348).
Sequence
MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLEL
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1358
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
28 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244) - Antibodies.comEnlarge Image

Western Blot - Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244)

Western blot analysis of extracts of various cell lines, using Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244)

Immunofluorescence analysis of NIH-3T3 cells using Anti-14-3-3 Theta/Tau Antibody [ARC1358] (A307244) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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