Anti-4-hydroxy-3-nitrophenylacetyl Chimeric Antibody [JW8/1] (A280785)

Anti-4-hydroxy-3-nitrophenylacetyl Chimeric Antibody [JW8/1]

Human chimeric monoclonal [JW8/1] antibody to 4-hydroxy-3-nitrophenylacetyl.

ELISA, Functional Assays

ELISA: 1:20 - 1:100

Human

Hapten, 4-hydroxy-3-nitrophenylacetyl (NP).

Human

Monoclonal

JW8/1

IgE

Tissue Culture Supernatant.

Liquid

Supplied as tissue culture supernatant with 0.2M TRIS/HCl, pH 7.4, 5-10% foetal calf serum and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

The immunoglobulin heavy chain has been produced by the linking of the antigen-binding, variable region genes of a mouse hybridoma to human constant region genes by in vitro DNA recombination procedures. The resulting chimeric antibody is subsequently expressed by the myeloma cell-line J558L after transfection. (The J558L cell-line self secretes a lambda light chain but no heavy chain). Thus a chimeric human IgE antibody specific for NP has been produced.