Anti-5 Lipoxygenase / 5-LO (phospho Ser523) Antibody (A283142)

Anti-5 Lipoxygenase / 5-LO (phospho Ser523) Antibody

Rabbit polyclonal antibody to 5 Lipoxygenase / 5-LO (phospho Ser523).

This antibody recognises 5-lipoxygenase (5-LO), also known as arachidonate 5-lipoxygenase, when phosphorylated at serine 523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LTs) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LTs include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.

WB: 1:1,000

Human, Primate, Rat

Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes the phosphorylated residue Ser523.

Rabbit

Polyclonal

IgG

Detects a double band of approximately 80 kDa in rat cortex lysate.

Liquid

Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti 5-lipoxygenase (pSer523) antibody recognizes 5-lipoxygenase (5-LO), also known as arachidonate 5-lipoxygenase, when phosphorylated at serine 523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LTs) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LTs include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.