Rabbit polyclonal antibody to 53BP1 (Phospho-Ser25)
Specificity
The antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 25.
Applications
IHC, IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 25 (E-D-S(p)-Q-P) derived from Human 53BP1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody #12125. The picture on the right is treated with the synthesized peptide.
Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody #12125. The picture on the right is treated with the synthesized peptide.
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