Anti-5HT2A Receptor Antibody (A82980)

5HT2A Receptor expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-5HT2A Receptor Antibody (A82980) at 2µg/ml and revealed with horseradish peroxidase (HRP).

Negative control for 5HT2A Receptor expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

5HT2A Receptor expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-5HT2A Receptor Antibody (A82980) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

5HT2A Receptor expression in A549 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-5HT2A Receptor Antibody (A82980) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.