Western blot analysis of extracts of various cell lines, using Anti-5T4 Antibody (A90048) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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Human monoclonal antibody to 5T4 Research grade Anti-Human 5T4 [PF-06263507] biosimilar for ELISA, Flow Cytometry, Functional Studies and in vivo Research.
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