Anti-AATF / DED Antibody [1B2D8] (A320269)

Anti-AATF / DED Antibody [1B2D8]

Rat monoclonal [1B2D8] antibody to AATF / DED.

This antibody recognises apoptosis-antagonizing transcription factor, also known as Rb-binding protein che-1.AATF was first identified as an RNA pol II – interacting factor. AATF is a protein encoded by the AATF gene. AATF mediates cellular responses and promotes cell proliferation and survival by inducing autophagy, DNA repair, and cell cycle arrest and inhibiting apoptosis. DNA damage leads to phosphorylation of AATF at tyrosine 366 by the MAP kinase-activated protein kinase 2 (MK2) (Höpker et al. 2012). Rat anti Mouse AATF antibody detects a band of 80 kDa.

IF, WB

IF: 1:100 - 1:500, WB: 2 µg/ml - 4 µg/ml

Mouse, Human

A peptide corresponding to amino acids 1-39 of mouse AATF (apoptosis-antagonizing transcription factor).

MAAPQPLALQLEQLLNPRPREADPEADPEEATRARVIDR

Rat

Monoclonal

1B2D8

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.035% Sodium Azide and 30% Glycerol.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse AATF antibody, clone 1B2D8 recognizes apoptosis-antagonizing transcription factor, also known as Rb-binding protein che-1.AATF was first identified as an RNA pol II – interacting factor (Fanciulli et al. 2000). AATF is a protein encoded by the AATF gene. AATF mediates cellular responses and promotes cell proliferation and survival by inducing autophagy, DNA repair, and cell cycle arrest and inhibiting apoptosis (Kumar et al. 2019). DNA damage leads to phosphorylation of AATF at tyrosine 366 by the MAP kinase-activated protein kinase 2 (MK2) (Höpker et al. 2012).Rat anti Mouse AATF antibody detects a band of 80 kDa.