Anti-Acetylated Lysine Antibody (A305219)

Western blot analysis of mouse Spleen lysates showing detection of Acetylated Lysine protein using Anti-Acetylated Lysine Antibody (A305219) at 1:1,000.

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Acetylated Lysine Antibody (A305219), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Nucleus. Cytoplasm. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of Bovine serum albumin showing detection of Acetylated Lysine protein using Anti-Acetylated Lysine Antibody (A305219) at 1:1,000. Acetylated lysine in BSA (Left) and Acetylated BSA (Right).

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Acetylated Lysine Antibody (A305219), at 1:100 for 12 hours at 4°C. The secondary antibody used was R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Nucleus. Cytoplasm. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Acetylated Lysine Antibody. (C) Composite. Heat Shocked at 42°C for 1h.