Anti-ACK1 Antibody (A14035)

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This product is discontinued

Anti-ACK1 Antibody (A14035) has been discontinued and is no longer available.

View all ACK1 Antibodies.

Specifications

Name

Anti-ACK1 Antibody

Description

Rabbit polyclonal antibody to ACK1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 920-1038 of human TNK2 (NP_005772.3).

Sequence

KANFSTNNSNPGARPPPPRATARLPQRGCPGDGPEAGRPADKIQMAMVHGVTTEECQAALQCHGWSVQRAAQYLKVEQLFGLGLRPRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHKR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

115 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ACK-1, Activated CDC42 kinase 1, TNK2, Tyrosine kinase non-receptor protein 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-ACK1 Antibody (A14035)

Western blot analysis of extracts of various cell lines, using Anti-ACK1 Antibody (A14035) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Immunohistochemistry - Anti-ACK1 Antibody (A14035)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-ACK1 Antibody (A14035) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ACK1 Antibody (A14035)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ACK1 Antibody (A14035) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ACK1 Antibody (A14035)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-ACK1 Antibody (A14035) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.