This antibody reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa), a typical serine proteinase with trypsin-like specificity. Acrosin is stored in the acrosome of undamaged spermatozoa.
This antibody does not cross react with Dog, Cow or Human.
Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.
Host
Mouse
Clonality
Monoclonal
Clone ID
ACR-2
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
35 kDa, 45 kDa, 53 kDSa, 55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunocytochemistry staining of capacitated boar sperm. Acrosome visualized using Anti-Acrosin Antibody [ACR-2] (A86140), (purified; secondary antibody labeled with FITC, green), DNA stained with DAPI (blue).
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