Anti-Adenine Nucleotide Translocator 1/ANT 1 Antibody (A13720)

Western blot analysis of extracts of various cell lines, using Anti-Adenine Nucleotide Translocator 1/ANT 1 Antibody (A13720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Western blot analysis of extracts of MCF7 cells, using Anti-Adenine Nucleotide Translocator 1/ANT 1 Antibody (A13720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Western blot analysis of extracts from normal (control) and SLC25A4/ANT1 knockout (KO) 293T cells, using Anti-Adenine Nucleotide Translocator 1/ANT 1 Antibody (A13720) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of NIH/3T3 cells using Anti-Adenine Nucleotide Translocator 1/ANT 1 Antibody (A13720) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).