Rabbit polyclonal antibody to AFX (Phospho-Ser197)
Specificity
The antibody detects endogenous level of AFX only when phosphorylated at serine 197.
Applications
WB, IHC, ICC/IF
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 197 (A-A-S(p)-M-D) derived from Human AFX.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
AFX1, Fork head domain transcription factor AFX1, Forkhead box protein O4, MLLT7
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AFX (Phospho-Ser197) Antibody #11137 (left) or the same antibody preincubated with blocking peptide (right).
