AIRE expression in Human Spleen lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-AIRE Antibody (A84291) at 0.3µg/ml and detected by chemiluminescence.
AIRE expression in HEK293 cell lysate transfected with Human AIRE (Lane 6) or Mouse AIRE (Lane 7) and untransfected (Lane 5 and 7) analyzed by western blot. Staining was performed with Anti-AIRE Antibody (A84291) at 0.05µg/ml.
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