Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96257)

Name: Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody
Brand: Antibodies.com
SKU: A96257
Price: 115.0 GBP
Availability: InStock

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This product is discontinued

Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96257) has been discontinued and is no longer available.

View all available products.

Specifications

Name

Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody

Description

Rabbit polyclonal antibody to AKT1 + AKT3 (phospho Tyr437 + Tyr434).

Specificity

This antibody detects endogenous levels of AKT1/3 only when phosphorylated at Tyr437/434.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500~1:1000, ELISA: 1:10000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human AKT1/3 around the phosphorylation site of Tyr437/434 (amino acids 406-455).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.

Molecular Weight

55kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96257)

Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' using Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunohistochemistry - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96257)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.