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Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96258)

ELISA - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (P12-1074) - Antibodies.com Western Blot - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (P12-1074) - Antibodies.com
This product is discontinued

Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96258) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody
Description
Rabbit polyclonal antibody to AKT1 + AKT3 (phospho Tyr437 + Tyr434).
Specificity
This antibody detects endogenous levels of AKT1/AKT3 only when phosphorylated at Tyr437/Tyr434.
Applications
WB, IHC, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic phospho-peptide derived from human AKT1/3 around the phosphorylation site of Tyr437/Tyr434.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
55kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

ELISA - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (P12-1074) - Antibodies.comEnlarge Image

Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96258)

ELISA for immunogen phosphopeptide (left) and non-phosphopeptide (right) using Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody.
Western Blot - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (P12-1074) - Antibodies.comEnlarge Image

Western Blot - Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody (A96258)

Western blot analysis of lysates from K562 cells treated with insulin 0.01U/ml 15' using Anti-AKT1 + AKT3 (phospho Tyr437 + Tyr434) Antibody.