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Anti-AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) Antibody (A307481)

Western Blot - Anti-AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) Antibody (A307481) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) Antibody
Description
Rabbit polyclonal antibody to AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305).
Applications
WB
Dilutions
WB: 1:500-1:1,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around T308 of human AKTAKT1 (NP_005154.2).
Sequence
MKTFC
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) Antibody (A307481) - Antibodies.comEnlarge Image

Western Blot - Anti-AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) Antibody (A307481)

Western blot analysis of extracts of NIH/3T3 cells, using Anti-AKT1 (phospho Thr308) + AKT2 (phospho Thr309) + AKT3 (phospho Thr305) Antibody (A307481) at 1:1,000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.
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