Anti-AKT1 (phospho Tyr315) + AKT2 (phospho Tyr316) + AKT3 (phospho Tyr312) Antibody (A283549)

Anti-AKT1 (phospho Tyr315) + AKT2 (phospho Tyr316) + AKT3 (phospho Tyr312) Antibody

Rabbit polyclonal antibody to AKT1 (phospho Tyr315) + AKT2 (phospho Tyr316) + AKT3 (phospho Tyr312).

This antibody recognises AKT1 when phosphorylated at tyrosine 315, AKT2 when phosphorylated at tyrosine 316 and AKT3 when phosphorylated at tyrosine 312. AKT 1, 2 and 3 are serine/threonine protein kinases that play a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. AKT1 is phosphorylated at tyrosine 315 (corresponding to tyrosine 316/312 of AKT2/AKT3 respectively) by Src and PTK6, tyrosine phosphorylation is important for growth factor mediated activation of AKT.

WB: 1:500 - 1:1,000

Human, Mouse

Phospho-specific peptide corresponding to residues surrounding Tyr315 of human AKT1/ Tyr316 of human AKT2/ Tyr312 of human AKT3.

Rabbit

Polyclonal

IgG

Lot Specific

Liquid

Supplied in Phosphate Buffered Saline with 0.05% ProClin 300 and 50% Glycerol.

Store undiluted at -20°C. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti AKT1 (pTyr315)/AKT2 (pTyr316)/AKT3 (pTyr312) antibody recognizes AKT1 when phosphorylated at tyrosine 315, AKT2 when phosphorylated at tyrosine 316 and AKT3 when phosphorylated at tyrosine 312. AKT 1, 2 and 3 are serine/threonine protein kinases that play a key role in the PI3K-Akt signaling pathway, known to regulate cell growth, proliferation and survival. AKT1 is phosphorylated at tyrosine 315 (corresponding to tyrosine 316/312 of AKT2/AKT3 respectively) by Src and PTK6, tyrosine phosphorylation is important for growth factor mediated activation of AKT (Zheng et al. 2010).