Specificity
The epitope for this antibody is located in the peptide KYEGSGEDGGA, amino acids 342-352 of the human Aldolase C. Interestingly, this antibody does not cross-react with mouse Aldolase C, which is somewhat different in sequence, RYEGSGDGGA, with a R>K change and missing an E residue, and since this antibody binds to aldolases from other species also lacking the E residue, and since the K>R change is unique to mouse, we can conclude that the K342 is essential for the binding of this antibody.