Mouse monoclonal [W8B2B10] antibody to Alkaline Phosphatase (APC).
Specificity
This antibody recognizes TNAP, an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 2-5 µg/ml.
Reactivity
Canine, Porcine, Sheep, Monkey, Goat, Human
Immunogen
WERI-RB-1 retinoblastoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
W8B2B10
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of TNAP positive HeLa cells (red-filled) from TNAP negative HeLa cells (black-dashed) in flow cytometry surface staining of HeLa cellular suspension, labeling Alkaline Phosphatase with Anti-Alkaline Phosphatase Antibody [W8B2B10] (APC) (A121846), (concentration in sample 0.56 µg/ml).
Flow cytometry surface staining patterns of HeLa cells stained using Anti-Alkaline Phosphatase Antibody [W8B2B10] (APC) (A121846), (concentration in sample 0.56 µg/ml, left) or Mouse IgG1 kappa Isotype Control [MOPC-21] (APC) (A86198), (concentration in sample 0.56 µg/ml, same as TNAP APC concentration, right).
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Proteins predicted to interact with Alkaline Phosphatase
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-Alkaline Phosphatase Antibody [W8B2B10] (APC) (A121846)](https://cdn.antibodies.com/image/catalog/121/A121846_1.jpg?profile=product_top)
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![Flow Cytometry - Anti-Alkaline Phosphatase Antibody [W8B2B10] (APC) (A121846)](https://cdn.antibodies.com/image/catalog/121/A121846_1.jpg?profile=product_image)
![Flow Cytometry - Anti-Alkaline Phosphatase Antibody [W8B2B10] (APC) (A121846)](https://cdn.antibodies.com/image/catalog/121/A121846_2.jpg?profile=product_image)