Rabbit polyclonal antibody to ALOX5 (Phospho-Ser523)
Specificity
The antibody detects endogenous levels of ALOX5 only when phosphorylated at serine523.
Applications
WB
Reactivity
Human, Rat
Immunogen
Peptide sequence around phosphorylation site of serine 523 (R-K-S(p)-K-G) derived from Human ALOX5.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from Jurkat cells, using ALOX5 (Phospho-Ser523) antibody #12128. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2), using ALOX5 (Phospho-Ser523) Antibody #12128. The lane on the left is treated with synthesized peptide.
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