Anti-alpha 2 Macroglobulin Antibody [M5] (Biotin) (A281003)

Anti-alpha 2 Macroglobulin Antibody [M5] (Biotin)

Mouse monoclonal [M5] antibody to alpha 2 Macroglobulin (Biotin).

This antibody recognises alpha-2-macroglobulin (A2M), also known as CPAMD5. A2M is a major proteinase inhibitor, with the ability to bind most proteinases and many growth factors, hormones and cytokines in human blood and tissue. A2M is largely produced by the liver under conditions of stress, and is then secreted into the blood and extracellular spaces. It is also produced locally by macrophages, fibroblasts, and epithelial cells. A2M levels dramatically decrease as humans age, a process which has been hypothesized to facilitate tumor development. In naked mole rats, a species with pronounced resistance to cancer, A2M has been shown to inhibit tumor growth regardless of tumor origin. The biotinylated Mouse anti Human A2M antibody, clone M5 (MCA6217B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human A2M antibody, clone M17 (MCA6216GA) as the capture antibody.

ELISA: 1 µg/ml

Human

E. coli derived recombinant human A2M (Ser24-Ala1474).

Mouse

Monoclonal

M5

IgG1

Protein G affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human A2M antibody, clone M5, recognizes alpha-2-macroglobulin (A2M), also known as CPAMD5. A2M is a major proteinase inhibitor, with the ability to bind most proteinases and many growth factors, hormones and cytokines in human blood and tissue (Kurz et al. 2017). A2M is largely produced by the liver under conditions of stress, and is then secreted into the blood and extracellular spaces. It is also produced locally by macrophages, fibroblasts, and epithelial cells (Kim et al. 2017). A2M levels dramatically decrease as humans age, a process which has been hypothesized to facilitate tumor development. In naked mole rats, a species with pronounced resistance to cancer, A2M has been shown to inhibit tumor growth regardless of tumor origin (Kurz et al. 2017).The biotinylated Mouse anti Human A2M antibody, clone M5 (MCA6217B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human A2M antibody, clone M17 (MCA6216GA) as the capture antibody.

A2M, Alpha-2-M, Alpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, CPAMD5

This product is for research use only. It is not intended for diagnostic or therapeutic use.