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Anti-alpha A Crystallin/CRYAA Antibody (A16759)

Western Blot - Anti-alpha A Crystallin/CRYAA Antibody (A16759) - Antibodies.com Immunofluorescence - Anti-alpha A Crystallin/CRYAA Antibody (A16759) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to alpha A Crystallin/CRYAA for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-alpha A Crystallin/CRYAA Antibody
Description
Rabbit polyclonal antibody to alpha A Crystallin/CRYAA.
Applications
WB, ICC/IF
Dilutions
WB: 1:2,000-1:6,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat, Zebrafish
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA (NP_000385.1).
Sequence
MDVTIQHPWFKRTLGPFYPSRLFDQFFGEGLFEYDLLPFLSSTISPYYRQSLFRTVLDSGISEVRSDRDKFVIFLDVKHFSPEDLTVKVQDDFVEIHGKHNERQDDHGYISREFHRRYRLPSNVDQSALSCSLSADGMLTFCGPKIQTGLDATHAERAIPVSREEKPTSAPSS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
19 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-alpha A Crystallin/CRYAA Antibody (A16759) - Antibodies.comEnlarge Image

Western Blot - Anti-alpha A Crystallin/CRYAA Antibody (A16759)

Western blot analysis of HepG2, using Anti-alpha A Crystallin/CRYAA Antibody (A16759) at 1:5,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-alpha A Crystallin/CRYAA Antibody (A16759) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-alpha A Crystallin/CRYAA Antibody (A16759)

Immunofluorescence analysis of rat Eye using Anti-alpha A Crystallin/CRYAA Antibody (A16759) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
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