Mouse monoclonal [1A4] antibody to alpha Smooth Muscle Actin (CF®594).
Applications
Flow Cytometry, IF, IHC, IHC-P
Positive Control
Blood vessels in normal tissues, smooth muscle, or leiomyosarcoma.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL, IHC-P: 0.25-0.5 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
1A4
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®594
Concentration
0.1 mg/mL
Predicted MW
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-alpha Smooth Muscle Actin Antibody [1A4] (CF®594) (A352703)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-alpha Smooth Muscle Actin Antibody [1A4] (CF®594) (A352703)