Mouse monoclonal [1A4] antibody to alpha Smooth Muscle Actin (CF®680R).
Applications
Flow Cytometry, IF, IHC, IHC-P
Positive Control
Blood vessels in normal tissues, smooth muscle, or leiomyosarcoma.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 0.5-1 µg/mL, IHC-P: 0.25-0.5 µg/mL. For IHC-P, heat-induced epitope retrieval is recommended using 10 mM citrate buffer, pH 6.0, for 10-20 minutes, followed by cooling to room temperature for 20 minutes. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
1A4
Isotype
IgG2a
Light Chains
kappa
Conjugate
CF®680R
Concentration
0.1 mg/mL
Predicted MW
42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Publishing research using Anti-alpha Smooth Muscle Actin Antibody [1A4] (CF®680R) (A352701)? Please let us know so that we can list the citation on this page.
Proteins predicted to interact with alpha Smooth Muscle Actin
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.