Western Blot - Anti-alpha smooth muscle Actin Antibody (A89813)
Western blot analysis of Mouse heart, using Anti-alpha smooth muscle Actin Antibody (A89813) at 1:800 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophagus using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil (negative control sample) using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat rectum cells using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of human smooth muscle cells using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of mouse colon cells using Anti-alpha smooth muscle Actin Antibody (A89813) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [1A4] (A249894) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249894_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [1A4] - BSA and Azide free (A253074) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253074_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [SPM332] (A249895) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249895_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [SPM332] - BSA and Azide free (A253075) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253075_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [ACTA2/791] (A249896) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249896_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [1A4 + ACTA2/791] (A249897) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249897_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [ACTA2/791] - BSA and Azide free (A253076) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253076_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody [1A4 + ACTA2/791] - BSA and Azide free (A253077) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253077_1.jpg?profile=product_alternative)
