Recombinant full-length human alpha Synuclein protein, region 61-95 corresponding to the central “non-amyloid beta component of Alzheimer’s disease amyloid”, expressed in and purified from E. coli.
Host
Chicken
Clonality
Polyclonal
Isotype
IgY
Conjugate
Unconjugated
Purification
Immunogen affinity purification.
Concentration
1 mg/ml
Molecular Weight
15 kDa (by SDS-PAGE)
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Storage
Shipped at 4°C. Store at +4°C. Do not freeze!
Synonyms
Alpha-synuclein, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, PARK1, SNCA
Immunofluorescent analysis of a section of rat cerebellum stained with Anti-Alpha-synuclein Antibody (A85289), dilution 1:3,000, in red, and co-stained with Anti-GFAP Antibody (A85419), dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with above antibodies. The alpha-synuclein protein is concentrated in presynaptic regions in the granular and molecular layers, while the GFAP antibody stains the network of Bergmann and astroglial cells.
Western Blot - Anti-alpha Synuclein Antibody (A85289)
Western blot analysis of different tissue lysates using Anti-Alpha-synuclein Antibody (A85289), dilution 1:2,000 in green. The lanes contain: [Lane 1] protein standard (red), [Lane 2] rat brain, [Lane 3] rat spinal cord, [Lane 4] mouse brain, and [Lane 5] mouse spinal cord. The strong band at about 15 kDa corresponds to the alpha-synuclein protein in brain extracts, which are rich in synapses, while a weaker band is seen in spinal cord extracts where synapses are a more minor component.
Mixed rat neuron-glial cultures stained with Anti-Alpha-Synuclein Antibody (red) and Anti-MAP2 Antibody (green). The alpha-synuclein antibody stains vesicular structures - the perikarya and processes of the neurons in this image. Note that some of the neuronal perikarya contain much more alpha-synuclein than others. The blue channel shows the localization of DNA.
Immunohistochemistry analysis of a formalin fixed paraffin embedded human cerebellum section with Anti-alpha Synuclein Antibody (A85289) at a dilution of 1:5,000 detected in DAB (brown) following the ABC method. Counterstained with Hematoxylin (blue). The a-synuclein protein is concentrated in synapses, in particular highlighting the glomeruli synapses in the granule layer and those on Purkinje dendrites in the molecular layer.
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