Mouse monoclonal [TU-16] antibody to alpha Tubulin.
Specificity
This antibody reacts with alpha-tubulin of all tested species, under denaturing and non-denaturing conditions.
Applications
IP, WB, IHC-P, ICC, ELISA
Dilutions
IHC-P: 10 µg/ml, IP: Reducing conditions, WB: 1-2 µg/ml. This antibody can be used for Western blotting, but its alternative TU-02 (11-447-C100) gives better signal in this application.
Reactivity
Mouse, Rat, Porcine, Nicotiana, Human, Canine
Immunogen
Porcine brain microtubule protein MTP-1.
Host
Mouse
Clonality
Monoclonal
Clone ID
TU-16
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by Anti-alpha Tubulin Antibody [TU-16] and its detection by Anti-alpha Tubulin Antibody [TU-01]. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa. L = lysate. IPr = immunoprecipitate (reducing conditions).
![Immunoprecipitation - Anti-alpha Tubulin Antibody [TU-16] (A86708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86709_829.jpg?profile=product_top)
![Immunoprecipitation - Anti-alpha Tubulin Antibody [TU-16] (A86708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86709_829.jpg?profile=product_top_thumb)
![Immunoprecipitation - Anti-alpha Tubulin Antibody [TU-16] (A86708) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86709_829.jpg?profile=product_image)
![Immunocytochemistry - Anti-alpha Tubulin Antibody [TU-01] (A86726) - Antibodies.com](https://cdn.antibodies.com/image/catalog/86/A86726_840.jpg?profile=product_alternative)
![Western Blot - Anti-alpha Tubulin Antibody [ARC2486] (A308887) - Antibodies.com](https://cdn.antibodies.com/image/catalog/308/A308887_1.jpg?profile=product_alternative)
![Immunocytochemistry - Anti-alpha Tubulin Antibody [RM113] (A121300) - Antibodies.com](https://cdn.antibodies.com/image/catalog/121/A121506_1.png?profile=product_alternative)
