Western Blot - Anti-Amino-Terminal Enhancer of Split/AES Antibody (A83048)
Amino-Terminal Enhancer of Split/AES expression in Human Brain (Cerebral Cortex) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Amino-Terminal Enhancer of Split/AES Antibody (A83048) at 0.3µg/ml and detected by chemiluminescence.
Immunohistochemistry - Anti-Amino-Terminal Enhancer of Split/AES Antibody (A83048)
Amino-Terminal Enhancer of Split/AES expression in Human Colon analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Amino-Terminal Enhancer of Split/AES Antibody (A83048) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
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