Western Blot - Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A16652)
Western blot analysis of extracts of 293T cells, using Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A16652) at 1:1,000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A16652)
Western blot analysis of extracts of C6 cells, using Anti-AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) Antibody (A16652) at 1:1,000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
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