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Anti-AMPK beta 2/PRKAB2 Antibody (A15447)

Western Blot - Anti-AMPK beta 2/PRKAB2 Antibody (A15447) - Antibodies.com Western Blot - Anti-AMPK beta 2/PRKAB2 Antibody (A15447) - Antibodies.com Immunofluorescence - Anti-AMPK beta 2/PRKAB2 Antibody (A15447) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to AMPK beta 2/PRKAB2 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-AMPK beta 2/PRKAB2 Antibody
Description
Rabbit polyclonal antibody to AMPK beta 2/PRKAB2.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human PRKAB2 (NP_005390.1).
Sequence
MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQ
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
33 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-AMPK beta 2/PRKAB2 Antibody (A15447) - Antibodies.comEnlarge Image

Western Blot - Anti-AMPK beta 2/PRKAB2 Antibody (A15447)

Western blot analysis of extracts of various cell lines, using Anti-AMPK beta 2/PRKAB2 Antibody (A15447) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-AMPK beta 2/PRKAB2 Antibody (A15447) - Antibodies.comEnlarge Image

Western Blot - Anti-AMPK beta 2/PRKAB2 Antibody (A15447)

Western blot analysis of extracts of Jurkat cells, using Anti-AMPK beta 2/PRKAB2 Antibody (A15447) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-AMPK beta 2/PRKAB2 Antibody (A15447) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-AMPK beta 2/PRKAB2 Antibody (A15447)

Immunofluorescence analysis of NIH/3T3 cells using Anti-AMPK beta 2/PRKAB2 Antibody (A15447) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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