Rabbit polyclonal antibody to AMPKα1 / AMPKα2 (Phospho-Thr183 / Thr172)
Specificity
The antibody detects endogenous level of AMPKα1 / AMPKα2 only when phosphorylated at threonine 183 or 172.
Applications
WB, IHC
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 183(L-R-T(p)-S-C) / threonine172(L- R-T(p)-S-C) derived from Human AMPKα1 / AMPKα2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from C2C12 cells, treated with serum or calf intestinal phosphatase (CIP), using AMPKα1/AMPKα2 (Phospho-Thr174/Thr172) Antibody #11183.
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