Rabbit polyclonal antibody to Amyloid β A4 (Phospho-Thr743 / 668)
Specificity
The antibody detects endogenous levels of Amyloid β A4 only when phosphorylated at threonine 743 / 668.
Applications
WB, IHC, ICC/IF
Dilutions
Western blotting: 1:500~1:1000 Immunohistochemistry: 1:50~1:100 Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine743 / 668 (A-V-T(p)-P-E) derived from Human Amyloid β A4.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0 mg/mL
Formulation
Supplied in phosphate-buffered saline (without Mg²? and Ca²?), pH 7.4, containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western blot analysis of extracts from cos-7 cells (Lane 2), using Amyloid β A4 (Phospho-Thr743/668) Antibody #11643. The lane on the left is treated with antigen-specific peptide.
Immunohistochemical analysis of paraffin-embedded human brain tissue using Amyloid β A4 (phospho-Thr743/668) antibody #11643 (left)or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed HeLa cells using Amyloid β A4 (phospho-Thr743/668) Antibody #11643.
Publishing research using Anti-Amyloid β A4 (Phospho-Thr743 / 668) Antibody (A50126)? Please let us know so that we can list the citation on this page.
