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Anti-Annexin V/ANXA5 Antibody (A13619)

Western Blot - Anti-Annexin V/ANXA5 Antibody (A13619) - Antibodies.com Western Blot - Anti-Annexin V/ANXA5 Antibody (A13619) - Antibodies.com Immunofluorescence - Anti-Annexin V/ANXA5 Antibody (A13619) - Antibodies.com
Knockout Validated
$485
Product Datasheet

Rabbit polyclonal antibody to Annexin V/ANXA5 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Annexin V/ANXA5 Antibody
Description
Rabbit polyclonal antibody to Annexin V/ANXA5.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human Annexin A5/Annexin V (NP_001145.1).
Sequence
MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Annexin V/ANXA5 Antibody (A13619) - Antibodies.comEnlarge Image

Western Blot - Anti-Annexin V/ANXA5 Antibody (A13619)

Western blot analysis of extracts of various cell lines, using Anti-Annexin V/ANXA5 Antibody (A13619) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.
Western Blot - Anti-Annexin V/ANXA5 Antibody (A13619) - Antibodies.comEnlarge Image

Western Blot - Anti-Annexin V/ANXA5 Antibody (A13619)

Western blot analysis of extracts from normal (control) and Annexin A5/Annexin V knockout (KO) 293T cells, using Anti-Annexin V/ANXA5 Antibody (A13619) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunofluorescence - Anti-Annexin V/ANXA5 Antibody (A13619) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Annexin V/ANXA5 Antibody (A13619)

Immunofluorescence analysis of HeLa cells using Anti-Annexin V/ANXA5 Antibody (A13619) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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