Anti-Annexin V / ANXA5 Antibody [VAA-33] (Biotin) (A281899)

Anti-Annexin V / ANXA5 Antibody [VAA-33] (Biotin)

Mouse monoclonal [VAA-33] antibody to Annexin V / ANXA5 (Biotin).

This antibody recognises human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Mouse anti Human annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications.

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Human

Recombinant Annexin V.

Mouse

Monoclonal

VAA-33

Protein G affinity chromatography of tissue culture supernatant.

Lot Specific

Liquid

Supplied in TRIS Buffered Saline, 1% BSA and 0.02% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Human annexin V antibody, clone VAA-33 recognizes human Annexin V, a phospholipid binding protein which binds with high affinity and selectivity to phosphatidylserine (PS), in the presence of calcium. The translocation of PS from the cytosolic leaflet to the outer leaflet of the plasma membrane, due to a loss in plasma membrane asymmetry during apoptosis, acts as an in vivo signal for the removal of the dying cell by phagocytosis. Detection of PS using Annexin V therefore provides a reliable diagnostic tool for the indication of early programmed cell death. Annexin V has also been identified as an anticoagulant protein in the blood coagulation cascade, by acting as an inhibitor of prothrombin activation. The presence of antibodies to Annexin V, are associated with systemic lupus erythematosus (SLE), recurrent spontaneous abortions and systemic sclerosis (SSc). Mouse anti Human annexin V antibody, clone VAA-33 has been reported to work in Western blotting applications (Wedher et al. 2009).

This product is for research use only. It is not intended for diagnostic or therapeutic use.