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Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667)

Western Blot - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) - Antibodies.com Western Blot - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) - Antibodies.com Immunohistochemistry - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC0559] antibody to Antithrombin III/ATIII for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Antithrombin III/ATIII Antibody [ARC0559]
Description
Rabbit monoclonal [ARC0559] antibody to Antithrombin III/ATIII.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SERPINC1 (P01008).
Sequence
MYSNVIGTVTSGKRKVYLLSLLLIGFWDCVTCHGSPVDICTAKPRDIPMNPMCIYRSPEKKATEDEGSEQKIPEATNRRVWELSKANSRFATTFYQHLAD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0559
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
58 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) - Antibodies.comEnlarge Image

Western Blot - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667)

Western blot analysis of extracts of various cell lines, using Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Western Blot - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) - Antibodies.comEnlarge Image

Western Blot - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667)

Western blot analysis of extracts of various cell lines, using Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Antithrombin III/ATIII Antibody [ARC0559] (A80667) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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