Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591)

Name: Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998]
Brand: Antibodies.com
SKU: A307591
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC0998] antibody to AP-2 complex subunit alpha-1 for WB and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998]

Description

Rabbit monoclonal [ARC0998] antibody to AP-2 complex subunit alpha-1.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human AP2A1 (O95782).

Sequence

MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0998

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

100 - 108 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP2A1, CLAPA1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591)

Western blot analysis of extracts of various cell lines, using Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Immunofluorescence - Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591)

Immunofluorescence analysis of C6 cells using Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591)

Immunofluorescence analysis of NIH-3T3 cells using Anti-AP-2 complex subunit alpha-1 Antibody [ARC0998] (A307591) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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