Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CDC42 GTPase-activating protein, CDC42GAP, GTPase-activating protein rhoGAP, p50-RhoGAP, Rho GTPase-activating protein 1, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, RHOGAP1
Western blot analysis of extracts of various cell lines, using Anti-ARHGAP1 Antibody (A11677) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Immunoprecipitation analysis of 200µg extracts of HeLa cells using 3µg of Anti-ARHGAP1 Antibody (A11677). This Western blot was performed on the immunoprecipitate using Anti-ARHGAP1 Antibody (A11677) at a dilution of 1:1000.
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