Anti-ARHGEF5 Antibody (A88506)

Name: Anti-ARHGEF5 Antibody
Brand: Antibodies.com
SKU: A88506
Price: 270.0 GBP
Availability: InStock

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$355

Rabbit polyclonal antibody to ARHGEF5 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-ARHGEF5 Antibody

Description

Rabbit polyclonal antibody to ARHGEF5.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1248-1597 of human ARHGEF5 (NP_005426.2).

Sequence

QVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTALEFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLRQNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

176 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Ephexin-3, Guanine nucleotide regulatory protein TIM, Oncogene TIM, p60 TIM, Rho guanine nucleotide exchange factor 5, TIM, Transforming immortalized mammary oncogene

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-ARHGEF5 Antibody (A88506)

Western blot analysis of extracts of various cell lines, using Anti-ARHGEF5 Antibody (A88506) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Immunohistochemistry - Anti-ARHGEF5 Antibody (A88506)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-ARHGEF5 Antibody (A88506) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-ARHGEF5 Antibody (A88506)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-ARHGEF5 Antibody (A88506) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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