Anti-Arrestin 1 Antibody [S128] (A85410)

Immunohistochemistry analysis of a 4% PFA fixed paraffin embedded rat retina section with Anti-Arrestin 1 Antibody [S128] (A85410) at a dilution of 1:1,000 detected in DAB (brown) following the ImmPress method. Counterstained with Hematoxylin (blue). Anti-Arrestin 1 Antibody [S128] (A85410) strongly labeled the inner surface of the outer nuclear layer of the retina. Note: this antibody has not been tested in NBF fixed material.

Confocal image of a pig retina stained with Anti-Arrestin-1 Antibody (green). Visual arrestin is most abundant in the outer segments (OS) and inner surface of the outer nuclear layer (ONL), and can be used to identify components of rod photoreceptor cells. (Cone photoreceptors have a different arrestin isotype). Other retinal layers are inner segments (IS), outer plexiform layer (OPL), inner nuclear layer (INL) and inner plexiform layer (IPL). The red stain is from Anti-Fox2 Antibody which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).

Blot of bovine retinal extracts probed with Anti-Arrestin-1 Antibody. The antibody stains a band corresponding to retinal arrestin at about 48 kDa.